
Embryology Laboratory
An embryology laboratory is our best attempt to simulate the conditions where life begins in the female reproductive tract. Our embryology lab was designed from the collective research, knowledge, and experience that our team of experts has gleaned over the last 25+ years. The space is isolated from the outside world with tight seals, and the environment is pressurized, which allows strict control of temperature, humidity, and air purity.
The in vitro fertilization journey begins in a procedure room adjacent to the IVF lab, where eggs (oocytes) are removed from the patient’s ovaries with a thin ultrasound-guided needle while she is sedated. Patients typically go home within 60 minutes of their oocyte retrieval procedure. The harvested eggs are immediately transferred into a working incubator called an isolette. This allows our embryologist to identify the mature eggs under magnification and to prepare them for their introduction to sperm, a process called fertilization. There are essentially two types of fertilization.
Conventional Insemination vs. Intracytoplasmic Sperm Injection
Conventional Insemination involves placing oocytes into a drop of media in the presence of 50-100,000 sperm. Overnight the sperm compete and make their way through the cumulus cells and zona pellucida surrounding each oocyte. When the first sperm successfully enters the egg, it triggers a chemical reaction in the zona pellucida, preventing any other sperm from progressing. This ensures that each egg is only fertilized by one sperm.

Intracytoplasmic sperm injection (ICSI) is the second method of insemination, and it involves directly injecting a single sperm into each mature egg. This procedure was first introduced in the 1990s as a way of compensating for very low sperm counts or sperm with poor motility. Today there are many more indications for ICSI; ICSI inseminates most eggs in 2013. At Atlantic Reproductive, approximately 80% of the eggs that undergo ICSI continue to develop.

Embryo Biospy
At Atlantic Reproductive, all embryos are cultured to the blastocyst stage of development. Our consistently producing high-quality blastocysts allows us to “push” out to Day 5, improving our capacity to select the correct embryo for transfer. It also allows us to use the Select CCS platform for preimplantation genetic screening. Select CCS is a clinically validated method to screen all 24 chromosomes of the embryo using a small piece of the trophectoderm (future placenta).
At Atlantic Reproductive, we believe strongly in combining well-established clinical practices with the newest validated laboratory methods for our patients. Vitrification is a method of cryopreserving your oocytes or embryo development so they can be stored for future use. Vitrification is superior to the more commonly used slow-cooling method because it decreases the chances of cell damage, improving patient outcomes.
Fertility Preservation
Increasingly, assisted reproductive technologies to help individuals and couples preserve their fertility for later conception. Fertility Preservation can be accomplished in the embryology laboratory at Atlantic Reproductive by either vitrifying blastocysts, as described above, or gametes (sperm or eggs). Indications for gamete cryopreservation include patients who are considering medical therapies that may harm their ovaries or testicles or patients who wish to delay childbearing and stop their biological clock while looking for the right partner or pursuing important education or career opportunities.
